Dr. Tischler

Dr. Dirk Tischler

Dr. Dirk Tischler


Post doc/ Wissenschaftlicher Mitarbeiter

Leipziger Str. 29, Zimmer 150

Telefon +49 3731 392103
dirk [dot] tischleratioez [dot] tu-freiberg [dot] de



 

Publications/ Oral and Poster Presentations/ Book chapters, Proceedings, and Theses

 

Research interests

  • actinobacteria, mainly rhodococci (enrichment and degradation of aromatic compounds)
  • genetic work with high-GC strains (PCR, cloning and expression)
  • characterization of (flavin-) monooxygenases
  • proteinbiochemistry and bioanalytics
  • metagenome-techniques
  • metallophores and their (bio)synthesis

Research projects

  • Enzymatic sugar coupling: No-P (ERA-IB, SMWK)
  • Asymmetric biotransformation by styrene monooxygenases to produce active agents (SMWK)
  • Bacterial metallophores for solvent extraction: BakSolEx (BMBF)
    • Screening for metallophores and metal binding studies
    • Synthetic, bio-bases metallophores
    • Application for binding rare elements
  • dye degrading enzymes: azoreductases and dyp-type peroxidases
  • novel flavoproteins for biocatalysis
  • R&D: Microbial metallophores (BRAIN)
  • R&D: Demethylation of Flavonoides (Symrise)
  • R&D: Characterization of nitrilase enzymes (BAYER) - finished
  • R&D: Metagenomic approach to identify novel sulfoxidizing enzymes (BAYER) - finished
  • Natural and artificial fusion proteins: styrene monooxygenase (Max-Buchner-Research Scholarship, DECHEMA) - finished
  • Construction of optimized one-component monooxygenases (SLUG) - finished
    • Optimization of one-component styrene monooxygenases
    • Screening-systems for monooxygenases
    • Development of DNA-Shuffling procedures
  • Genomics and Transcriptomics in Geobiotechnology and White Biotechnology: GETGEOWEB (ESF)- finished
    • Microbial degradation of styrene; involved enzymes
    • Genome of Rhodococcus opacus 1CP
    • Metagenome of styrene degrading Actinobacteria

Courses

  • Toxicology (lecture)
  • Microbiology of fossil and regenerative energy resources (lab)
  • Biotechnological Production Processes (lecture, lab)
  • Databases for Applied Natural Science: Microbiology (part-lecture)
  • Enzymes: Purification, Characterization, Mechanisms (lecture)
  • Summer school: BioNanoTec

Curriculum vitae

2016 - ...Author of RÖMPP; topic Enzymes (Georg Thieme Verlag)
2016 - ...Member of the "Zukunftsforum Biotechnologie" DECHEMA
2015 - ...Commissioner for education: Applied Natural Sciences
2014 - ...Member of the Alumni PhD board of the Deutsche Bundesstiftung Umwelt (DBU)
2013Research internship at Wageningen University, The Netherlands
2012 - ...Planned Habilitation with Prof. Dr. Michael Schlömann
2011Fulbright Scholarship: research internship at San Francisco State University, California
2010By DBU- and, Freunde und Förder der TUBAF e.V. founded research internship at Wageningen University, The Netherlands
2008 - 2012Doctorate: at Environmental Microbiology Group, TUBAF, DBU-Scholarship (StSp: Nachhaltige Bioprozesse)
2007Exams: Dipl.-Nat., TUBAF
2005Study: Environmental Microbiology / Biotechnology, TUBAF
2002 - 2007Study: Applied Natural Science, TU Bergakademie Freiberg (TUBAF)
2001General qualification for university entrance (Abitur)
1989 - 2001School
1982Born in Rodewisch

Awards and Scholarships

  • 2014 to 2016 - Member of American Society for Microbiology's (ASM) Young Leader Circle (YLC); Chair of the YLC for the first period (2014/2015); Past-Chair of YLC (2015/2016)
  • 2014 - DAAD Postdoc Travel Grant
  • 2013 - Max-Buchner-Research Scholarship (DECHEMA)
  • 2013 - FEMS Congress Grant for Young Scientists for the 5th Congress of European Microbiologists, Leipzig, Germany
  • 2013 to 2016 - American Society for Microbiology's (ASM) first Young Ambassador to Germany
  • 2012 - Award for young researchers (Saxony) of the „Dresdner Gesprächskreis der Wirtschaft und der Wissenschaft e.V.“
  • 2012 - Ph.D award „Bernhard von Cotta Preis“ of the „Verein der Freunde und Förderer der TU Bergakademie Freiberg e.V.“
  • 2011 - Student Travel Grant Award of the American Society for Microbiology for the 111th General Meeting, New Orleans, Louisiana
  • 2011 - Fulbright scholarship
  • 2008 - Ph.D scholarship from the Deutsche Bundesstiftung Umwelt (DBU)

Memberships (research societies)

Theses

  • doctorate (2012): Styrene monooxygenases of Rhodococcus opacus 1CP - A representative of a new type of flavin monooxygenases with potential for white biotechnology.
  • diploma project (2007): Trehalose-6-phosphate synthases of Rhodococcus opacus 1CP: Expression, purification and characterisation.
  • student research project (2007): Transposon-mutagenesis for trehalose-tetraester synthesis in Rhodococcus erythropolis B7g.

Review- and Editorial process

  • since 2014 member of editorial team of JSM Enzymology and Protein Science
  • since 2013 member of editorial team of American Journal of Current Microbiology
  • since 2009 reviewer for journals in the fields of microbiology, biotechnology, and org. chemistry