Dipl. Nat. Scholtissek (geb. Riedel)
Dipl. Nat. Anika Scholtissek
Research Group Environmental Microbiology
Gustav-Zeuner-Str. 6
Telefon +49 3731 394151
anika [dot] scholtissek
ioez [dot] tu-freiberg [dot] de
Active research project:
Thesis project: "Thermostable ene-reductase for biocatalysis" (Prof. Dr. Michael Schlömann, Dr. Dirk Tischler)
The project is funded by the European Union (EU), the European Social Fund (ESF) and the Saxonian Government. It was obtained by Anika Riedel as personal research scholarship! We thank the funding bodies!
Ene-reductases are widely applied flavoproteins for the asymmetric reduction of activated C=C-bonds through a trans-hydrogenation reaction of relevant industrial chemicals. Characterization of novel biocatalysts regarding to their stability against temperature and organic solvents is from major interest for biocatalysis.
The aim of this project is the identification of novel `thermophilic-like` ene-reductases from organisms as the actinobacterium Rhodococcus opacus 1CP or the acidophilic iron-oxidizing betaproteobacterium Ferrovum myxofaciens JA12. Promising candidates identified via genome mining and subsequent multiple sequence alignment will be proceeded for cloning, overexpression in Escherichia coli BL21 and biochemical characterization.
Site-specific mutagenesis of the cloned genes shall raise the half-life of enzyme inactivation at respective temperatures and improve the tolerance towards organic co-solvents as well as changing the influence of different substrates for enzyme inhibition.
Biocatalysis and subsequent analysis of (enantiopure) products will be substantial part of this study.
Research interests
- Functional genome analysis (sequence analysis)
- Cloning, expression and site-directed mutagenesis
- Biotransformations
- (Bio)analytics (RP-HPLC, GC, gel filtration)
- Protein biochemistrys
Vitae
| 2006 - 2012 | Study: Applied Natural Science, TU Bergakademie Freiberg (TUBAF) | |
| 2009-2010 | Student assistant, Helmholtz-Zentrum Geesthacht, Institute for coastal research; Department of Environmental chemistry | |
| 2010-2011 | Research assistant, Yale University, Department of Molecular, Cellular and Developmental Biology (DAAD scholarship) | |
| 2011-2012 | Diploma project at Environmental Microbiology Group, TUBAF | |
| 2012 | Graduation: Dipl.-Nat. at Environmental Microbiology Group, TUBAF | |
| since 2012 | Ph.D student: at Environmental Microbiology Group, TUBAF, ESF-project: GETGEOWEB | |
| 2014-2015 | Maternaty protection and parental leave | |
| since 2015 | Ph.D student: at Environmental Microbiology Group, TUBAF, ESF-project: Thermostable ene-reductases for biocatalysis |
Theses
- Student research project (2009): Bestimmung der Denitrifizierungsraten in Sedimenten der südlichen Nordsee und im Elbeästuar.
- Diploma project (2012): One pot synthesis for fine chemicals - applying a styrene monooxygenase and a styrene oxide isomerase.
Memberships (research societies)
- Vereinigung für Allgemeine und Angewandte Mikrobiologie e.V. (VAAM)
- Gesellschaft Deutscher Chemiker (GDCh)
- DECHEMA (Sektion Biokatalyse)
- American Society for Microbiology (ASM)
- Deutscher Naturschutzbund (NABU)