BakSolEx
r4 – project – junior research group
Bacterial metallophores for solvent extraction
(Bakterielle Metallophore für die Solventextraktion)
Funding Identifier: 033R147
Duration: 2 Phases (1. Jan. 2015 to 31. Dec. 2017, and to 31. Oct. 2020)
Aim
The interdisciplinary junior research group BakSolEx aims on the identification of novel bio-based metallophores. Thus we study especially soil bacteria which produce these as secondary metabolites under certain conditions. We study the production from gene via protein machinery to the final product. On the other hand synthetic approaches are conducted to build nature-like metallophores. Thus, metallophores shall be produced by biotechnological or synthetic chemical approaches and further be analyzed by various spectroscopic methods for metal binding. Finally, the metallophores shall be applied to a selective metal extraction. Target metals are Ga, Co, Mo, Ag and V. For these metals novel selective methods on base of the metallophores shall be developed and allow to improve the situation on rare elements for industry.
Team
| Dr. Dirk Tischler | Junior Group Leader | spectroscopy |
| MSc. Marika Mehnert | PhD student | cultivation and screening |
| Dipl.-Nat. Thomas Heine | PhD student | genomics and strain evolution |
| MSc. Pierre Seidel (OC, Prof. M. Mazik) | PhD student | synthesis and structural studies |
| Dipl.-Nat. Ringo Schwabe | PhD student (Saxony, scholarship) | plant-siderophore interaction, |
| MSc. Gerardo Retamal (Chilie, Prof. G. Levican) | PhD student (exchange) | screening, resistance |
| open | ... | contact group leader |
Former members: Mareike Noffke (BSc.), Alina Achterhold (BSc.), Sabine Vater (BSc.), Janine Slesaczek (MSc.), Catherine O. Esuola (PhD), Diogo N. Proença (Postdoc-Training)
Publications
Articles and Reviews
- O. Wiche, D. Tischler, C. Fauser, J. Lodemann, H. Heilmeier (2017) Effects of citric acid and the microbial siderophore desferrioxamine B (DFO-B) on the mobility of germanium (Ge) and rare earth elements (REEs) in soil and uptake in Phalaris arundinacea. In. J. Phytoremediat. online first.
- C.O. Esuola, O.O. Babalola, T. Heine, R. Schwabe, M. Schlömann, D. Tischler (2016) Identification and characterization of a FAD-dependent putrescine N-hydroxylase (GorA) from Gordonia rubripertincta CWB2. J. Mol. Catal. B Enzym.
- C.O. Esuola, O.O. Babalola, T. Heine, M. Schlömann, D. Tischler, S.O.S Akinyemi (2016). Horticultural crops development: the importance of fine chemicals production from microbial enzymes. Acta Hortic. 1110, 7-12.
Books, Chapters and Proceedings
- M. Schlömann, S. Schopf, D. Tischler, M. Mühling (2016) Biohydrometallurgy - a research area being new for Freiberg with the relevance for metal winning and purification of mine waters. In Glanzlichter der Forschung, 134-147; Ed. by U. Groß, Freiberg.
Oral Presentation:
- R. Schwabe, B. Obst, M. Mehnert, D. Tischler, O. Wiche (2017) Applying bacterial metallophores to mobilize metals (Ga vs. Fe). Oral Presentation, 19th EGU Vienna.
- D. Tischler, T. Heine, J. Zimmerling, M. Oelschlägel, M. Schlömann (2017) Von der Funktion zur Sequenz. Oral Presentation, MIKAT Workshop, Dresden; Oral Short Presentation, Future Technologies, Dresden.
- D. Tischler, J. Qi, I. Bento, C.O. Esuola, T. Heine, A. Scholtissek, M. Mehnert, R. Schwabe, J. Zimmerling, M. Oelschlägel (2016) Oxidoreductases for biocatalysis - from screening to function. Oral Presentation, Invited Seminar, Philipps-Universität Marburg, Marburg.
- Gerardo Retamal M, Marika Mehnert, Ringo Schwabe, Michael Schlömann, Dirk Tischler, Gloria Levicán J. (2016) Arsenic-binding siderophores from arsenic-tolerant actinobacteria. Biohydrometallurgy '16, Falmouth, UK.
- Thomas Heine, Marika Mehnert, Ringo Schwabe, Dirk Tischler (2016) Bacterial siderophores: A perspective on the Junior Research Group: BakSolEx. 67. Berg- und Hüttenmännischer Tag, Freiberg.
- Dirk Tischler, Marika Mehnert, Sabine Vater, Vivian Schulz, Gerardo Retamal M., Ringo Schwabe, Thomas Heine (2016) Metallophores selectively bind metals. Invited Oral Presentation, UFZ Energy Days 2016, Leipzig.
- Dirk Tischler, Marika Mehnert, Vivian Schulz, Sabine Vater, Catherine O. Esuola, Thomas Heine, Ringo Schwabe (2016) Metallophores. 1. Congress on resource efficiency, Pforzheim.
- Marika Mehnert, Sabine Vater, Vivian Schulz, Dirk Tischler (2016) Screening soil bacteria and fungi for metallophores. 1. Congress on resource efficiency, Pforzheim. Best Presenter Award.
- Marika Mehnert, Sabine Vater, Dirk Tischler (2015) Screening of soil bacteria for metallophores. 5. Int. Sci. Conf. Applied Natural Sciences, Jasná, Low Tatras.
- Dirk Tischler, Marika Mehnert, Thomas Heine, Ringo Schwabe (2015) Bacterial solvent extraction. 66. Berg- und Hüttenmännischer Tag, Freiberg.
- Ringo Schwabe, Anne Ballmann, Michael Schlömann, Gloria Levican, Oliver Wiche, Kathrin Leppchen-Fröhlich, Michael Meyer, Dirk Tischler (2015) Detection and isolation of metallophores from Gordonia rubripertincta CWB2. Oral Presentation, Environmental Biotechnology Meeting, Gliwice.
Poster:
- D.N. Proença, T. Heine, M. Mehnert, P.V. Morais, D. Tischler (2017) Genomic identification and biochemical characterization of siderophores produced by endophytic bacteria isolated from Pinus pinaster affected by Pine Wilt Disease. Poster Presentation, ASM Microbe 2017, New Orleans.
- C.O. Esuola, J. Trögl, O.O. Babalola, M. Schlömann, D. Tischler (2017) Biodegradation of industrially polluted soil matrix. Poster 386/EEP, VAAM annual meeting, Würzburg.
- R. Schwabe, B. Obst, M. Mehnert, D. Tischler, O. Wiche (2017) Applying bacterial metallophores to mobilize metals (Ga vs. Fe). Poster Presentation, 2nd Congress on resource efficiency, Pforzheim.
- Catherine O. Esuola, Olubukola O. Babalola, Thomas Heine, Ringo Schwabe, Michael Schlömann, Dirk Tischler (2016) GorA attacks the soft nucleophilic terminal amino group of diamines. Poster at OxiZymes, Wageningen.
- Marika Mehnert, Gerardo Retamal M., Thomas Heine, Ringo Schwabe, Michael Schlömann, Gloria Levicán J., Dirk Tischler (2016) Metall (and metalloide)-binding of different metallophores from bacteria and fungi. Poster at ASM annual meeting, Boston.
- Thomas Heine, Mareike Noffke, Marika Mehnert, Ringo Schwabe, Dirk Tischler (2016) Structure prediction, biosynthesis and purification of a siderophore from a thermophilic bacterium. Poster, VAAM, Jena.
- Marika Mehnert, Thomas Heine, Sabine Vater, Dirk Tischler (2016) Screening of metallophores from bacteria and fungi for metal-affinity. Poster, VAAM, Jena.
- Catherine O. Esuola, Thomas Heine, Olubukola O. Babalola, Michael Schlömann, Dirk Tischler (2015) Identification and characterisation of a novel diamine monooxygenase from Gordonia sp. CBW2. Poster, VAAM, Marburg.
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